Transcriptional regulation of autophagic genes by fenugreek. Jurkat cells incubated with 250, 500 and 1000 μg/mL of fenugreek extract were quantitatively analyzed for transcripts of autophagic pathway genes beclin 1, ATG5 and LC3 by RT-PCR using β-actin as internal reference. Dose dependent increase in LC3 transcripts was found in cells incubated for 48 hr while no significant changes were observed for beclin 1 and ATG5.
Al-Daghri et al. BMC Complementary and Alternative Medicine 2012 12:202 doi:10.1186/1472-6882-12-202