NPC sphered cells showed different properties compared to parental cells. (A) NPC TW01 sphere grew in serum free non-adherent condition (B) Different cell shape of TW01 parental and sphere-derived cells were noted when cultured adherently in 10% serum for 48 h. Sphered cells present in mesenchymal shape. (C) Sphere-derived cells present more powerful migratory behavior after 12 h of scraping (Scale bars show 100 μm).
Lin et al. BMC Complementary and Alternative Medicine 2012 12:201 doi:10.1186/1472-6882-12-201