Effect of the aerial part of Cryptotaenia japonica Hassk. (CJ) methanol extract on LPS-induced NO and iNOS.A. Peritoneal macrophages were primed with IFN-γ (0.5 ng/ml) for 2 h and then stimulated with LPS (100 ng/ml) for 18 h. NO in the medium was detected by the Griess reaction. The expression of iNOS protein was analyzed by Western blotting. β-tubulin was loaded as an internal control. B, C: IFN-γ-sensitized cells were stimulated with LPS in the presence of the CJ methanol extract for 18 h. D: Cells were cultured with CJ methanol extract for 24 h and cell viability was determined using the MTS assay. Viability was presented as percent of control cells (0 μg/ml). Data represent mean ± SD of two to four independent assays. * denotes significant difference ( P < 0.05) from cells treated with LPS alone.
Kang et al. BMC Complementary and Alternative Medicine 2012 12:199 doi:10.1186/1472-6882-12-199