Inhibitory effect of EMM on the degradation of IκB-α and the activation of NF-κB in LPS-stimulated RAW 264.7 cells. Cells were incubated with various concentrations of EMM for 1 h, and then stimulated with LPS (1 μg/mL) for 30 min. Cytosolic and nuclear fractions were prepared and analyzed by Western blotting using corresponding antibodies. The results presented are representative of three independent experiments. ap < 0.05 indicates significant differences compared to the control group. bp < 0.05 indicates significant differences compared to the LPS-only treated group.
Joung et al. BMC Complementary and Alternative Medicine 2012 12:171 doi:10.1186/1472-6882-12-171