Effect of EMM on LPS-induced iNOS and COX-2 protein and mRNA expression in RAW 264.7 cells. (A) Cells were pretreated with indicated concentration of EMM for 1 h and stimulated with LPS (1 μg/mL) for 16 h. Forty microgram of proteins were subjected to 10% SDS-PAGE. The expression of iNOS, COX-2, and β-actin protein was detected by Western blotting using corresponding antibodies. (B) Cells were pretreated with EMM for 1 h and stimulated with LPS for 6 h, and then total RNA was prepared for RT-PCR. The results presented are representative of three independent experiments. ap < 0.05 indicates significant differences compared to the control group. bp < 0.05 indicates significant differences compared to the LPS-only treated group.
Joung et al. BMC Complementary and Alternative Medicine 2012 12:171 doi:10.1186/1472-6882-12-171