Effect of OY and fermented OYs on LPS-induced (A) TNF-α cytokine and (B) mRNA expression. RAW 264.7 cells pretreated with the indicated concentrations of OY or fermented OYs for 30 min before being incubated with LPS (200 ng/mL) for (A) 24 hours and (B) 6 hours, respectively. The level of TNF-α was measured by ELISA and the mRNA level was assessed by RT-PCR. (A) The data are mean ± SE values of duplicate determinations from three independent experiments. *p < 0.05 and **p < 0.005, were calculated from comparing with LPS-stimulated values. (B) The values in the LPS-stimulated cells without OY treatment was set to 1.0 and the differences by treatment were quantitated. The experiments were repeated at least three times, and similar results were obtained.
Oh et al. BMC Complementary and Alternative Medicine 2012 12:17 doi:10.1186/1472-6882-12-17