Effects of GLY on wound healing migration of VSMCs cells. Wound was introduced by scraping confluent cell layers with a pipet tip. VSMCs cells were incubated with GLY (0.0625, 0.125, 0.25 and 0.5 mg/mL) for 18 h, and the migration distances of cells were calculated. (A) Representative photographs of invading cells that received either control (15% FBS) or GLY treatment. (B) Migrated cells across the black lines were counted in six random fields from each treatment. The mean number of cells in the denuded zone is quantified by three independent experiments. (C). Effects of GLY on transwell migration assay vascular smooth muscle cells. VSMCs were incubated with GLY (0.0625, 0.125, 0.25 and 0.5 mg/mL) for 18 h, and (D). The transwell migration cells were calculated. Photos of the migration VSMCs cells were taken under a microscope (100-fold magnification). Mean value was significantly different from that of the control group (15% FBS): * p < 0.05,** p < 0.01.
Chien et al. BMC Complementary and Alternative Medicine 2012 12:137 doi:10.1186/1472-6882-12-137