Table 3

Effects of L.obtusiloba extract on basal and IGF-1-induced signal transduction via IGF-1R

Hep3B

SK-Hep1



IGF-1

L.obtusiloba extract

IGF-1 and L.obtusiloba extract

IGF-1

L.obtusiloba extract

IGF-1 and L.obtusiloba extract


pIGF-1R

4.81 ± 0.40*

0.73 ± 0.08*

2.21 ± 0.31*; #

22.3 ± 1.98*

0.81 ± 0.07

5.02 ± 0.60*; #

pAkt

2.96 ± 0.25*

1.02 ± 0.10

1.67 ± 0.19*; #

27.7 ± 2.84*

1.12 ± 0.09

19.3 ± 1.45*; #

pStat3

2.01 ± 0.18*

0.20 ± 0.07*

0.38 ± 0.21*; #

2.91 ± 0.22*

1.23 ± 0.20

1.18 ± 0.19#

pErk2

1.32 ± 0.14*

0.13 ± 0.03*

0.57 ± 0.07*; #

2.81 ± 0.32*

1.01 ± 0.10

1.82 ± 0.17*; #


HCC cells were treated with 100 μg/ml L.obtusiloba extract, 125 ng/ml IGF 1, a combination of both or remained untreated for 24 h. Phosphorylation of IGF-1R and its downstream targets Akt, Stat3 and Erk were studied in whole cell lysates by specific western-blot analysis as shown in representative blots in Fig. 3. Activation of the proteins was determined from densitometric assessment in comparison to total expression levels of the respective non-phosphorylated protein. Mean values ± SD in relation to untreated cells from three independent experiments. *P < 0.05 compared to the untreated control, # P < 0.05 compared to IGF-1-treated cells.

Freise et al. BMC Complementary and Alternative Medicine 2011 11:39   doi:10.1186/1472-6882-11-39

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