Proliferation, apoptosis and invasion of HCC cell lines treated with L.obtusiloba extract. (A) Cell cycle synchronized HepG2, Hep3B, Huh 7 and SK-Hep1 cells were treated with up to 200 μg/ml L.obtusiloba extract for 24 h. Cultures without L.obtusiloba extract served as controls. Proliferation was determined by [3H]-thymidine incorporation within the last 4 h of the culture. Mean values ± SD of three parallel measurements. (B) HCC cells were incubated with 100 μg/ml L.obtusiloba extract for 24 h. Cultures without additives or with 100 nM staurosporine served as negative or positive controls, respectively. Enzymatic activities of caspase-3/7 were determined from cell lysates by fluorogenic substrate conversion. Shown are the mean values ± SD of four parallel measurements. (C) HCC cells were allowed to invade membranes coated with basement collagen in the absence or presence of 100 μg/ml L.obtusiloba extract. After 24 h, transmigrated cells were stained with crystal violet and numbers were counted. Shown are the mean values ± SD of three independent experiments with four parallel measurements.*P < 0.05, **P < 0.01, ***P < 0.001.
Freise et al. BMC Complementary and Alternative Medicine 2011 11:39 doi:10.1186/1472-6882-11-39