Open Access Open Badges Research article

Evaluation of free-radical quenching properties of standard Ayurvedic formulation Vayasthapana Rasayana

Sourav Mukherjee, Nayana Pawar, Omkar Kulkarni, Bhagyashri Nagarkar2, Shrikant Thopte2, Akshay Bhujbal1 and Pankaj Pawar3*

Author Affiliations

1 Modern College for Arts, Science and Commerce, Shivajinagar, Pune - 411 005, Maharashtra, India

2 Rajiv Gandhi Institute for IT and Biotechnology, Bharati Vidyapeeth University, Pune-Satara Road, Pune - 411 046, Maharashtra, India

3 Interactive Research School for Health Affairs, Bharati Vidyapeeth University, Pune-Satara Road, Pune - 411 043, Maharashtra, India

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BMC Complementary and Alternative Medicine 2011, 11:38  doi:10.1186/1472-6882-11-38

Published: 12 May 2011



Cellular damage induced by free-radicals like Reactive Oxygen and Nitrogen Species (ROS and RNS) has been implicated in several disorders and diseases, including ageing. Hence naturally occurring anti-oxidant rich-herbs play a vital role in combating these conditions. The present study was carried out to investigate the in vitro free-radical quenching capacity of a known Ayurvedic poly-herbal formulation called Vayasthapana Rasayana.


Methanol extracts of Vayasthapana Rasayana formulation (VRF) were studied for in vitro total antioxidant activity along with phenolic content and reducing power. In vitro assays like DPPH, FRAP, ABTS scavenging to evaluate radical quenching potential were performed.


The formulation has shown 94% at 0.1 mg/ml DPPH free-radical scavenging activity as against 84% at 0.1 mg/ml for standard ascorbic acid (IC50 value 5.51 μg/ml for VRF and 39 μg/ml for standard). It has a significant higher ferric reducing potential also (OD 0.87 at 700 nm & 0.21 at 0.1 mg/ml for VRF and standard, respectively). The total phenolic content (gallic acid equivalent) of the VRF is 8.3 mg per g of dry mass. Total antioxidant capacity of the formulation, estimated by FRAP was 1150 ± 5 μM Fe(II)/g dry mass. ABTS radical scavenging activity of VRF was 69.55 ± 0.21% at 100 μg/ml concentration with a IC50 value of 69.87 μg/ml as against 9% and 95% by ascorbic acid and Trolox (at 70.452 μg/ml and 0.250 μg/ml concentrations, respectively).


In Indian traditional Ayurvedic system, use of VRF is in regular practice for mainly combating age-related disorders and diseases as many of the components of the Rasayana are known for their free-radical scavenging activity. This study has validated the potential use of VRF as an anti-oxidant to fight age-related problems.