Comparative cell invasion ability of human cutaneous squamous cell carcinoma cells. (A) Invasive ability of human epidermoid carcinoma cells (A431) and head and neck cutaneous squamous cell carcinoma cells (SCC13) were compared. Equal numbers of cells were subjected to cell invasion using standard Boyden chamber assay. Twenty-four h later, invasive cells were detected on the membrane after staining with crystal violet. (B) The number of invasive cells were counted and the results expressed as the mean number of invasive cells ± SD/microscopic field (n = 3). Significantly higher versus A431 cells, *P < 0.0001. (C) Time-dependent cell invasion potential of human head and neck cutaneous SCC cells. (D) The invasive cells were counted and the results expressed as the mean number of invasive cells ± SD/microscopic field. Significant higher invasion of cells versus 6 h time point, *P < 0.001; ¶P < 0.01.
Sun et al. BMC Complementary and Alternative Medicine 2011 11:134 doi:10.1186/1472-6882-11-134