Figure 4.

(A) Represents effect of flavonoidal fraction on intracellular calcium levels in mouse islets. Intracellular calcium levels were measured using fluorescent probe fura-2AM. Values are expressed as ± SEM in triplicates where α = p < 0.01, β = p < 0.01, δ = p < 0.01 when 16.7 mM glucose group was compared to its 10, 50, 100, 250 groups and ➊ = p < 0.01, ➋ = p < 0.01, ➌ = p < 0.01 when 11.1 mM glucose + IBMX group was compared to its 10, 50, 100, 250 groups. (B) Represents effect of flavonoidal fraction on MTT activity in mouse islets. Cultured islets were exposed to pancreatic β- cell specific toxin, streptozotocin (2 mM) along with flavonoidal fraction at various concentrations and assessed for cytotoxicity. Values are expressed as ± SEM in triplicates where • = p < 0.01, ■ = p < 0.001, ♦ = p < 0.0001 when control group was compared with rest of the groups and α = p < 0.01, β = p < 0.01, δ = p < 0.01 where diabetic group was compared to rest of the groups. (C) Represents effect of flavonoidal fraction on intracellular ROS activity in mouse islets. Intracellular ROS was assessed by fluorescent probe DCFH-DA. Values are expressed as ± SEM in triplicates where • = p < 0.01, ■ = p < 0.001, ♦ = p < 0.0001 when control group was compared with rest of the groups and α = p < 0.01, β = p < 0.01, δ = p < 0.01 when diabetic group was compared to rest of the groups.

Ansarullah et al. BMC Complementary and Alternative Medicine 2011 11:126   doi:10.1186/1472-6882-11-126
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