Table 6

Effect of RHP and GLGPG on gene expression in NHAC-kn cells

Gene

CTa

IL-1β

IL-1β + RHP (250 mg/L)

IL-1β + GLGPG (9.7 mg/L)

fold change

fold change

p valueb

fold change

p valueb


MMP-1

29.5

22.8

10.4

0.008

20.0

0.418

MMP-2

23.4

0.8

0.6

0.249

0.8

0.332

MMP-3

28.1

167

89

0.048

128

0.135

MMP-9

29.6

7.2

6.0

0.025

5.0

0.203

MMP-13

28.1

2.2

1.3

0.048

2.0

0.135

ADAMTS-4

29.5

20.0

21.7

0.460

14.6

0.103

ADAMTS-5

21.7

1.3

0.9

0.011

1.7

0.148

TIMP-1

17.2

1.2

0.9

0.052

1.1

0.215

Aggrecan

28.8

1.0

0.8

0.196

1.1

0.340

Collagen 1

18.0

0.7

0.5

0.05

0.8

0.30

COL2A1

22.2

1.0

0.5

0.137

1.2

0.325

MIP-2

29.9

441

110

0.082

626

0.147

MIP-3α

30.0

481

271

0.007

436

0.126

CCL5/RANTES

26.7

87

42.0

0.009

54

0.046

IL-8

26.3

799

168

0.002

760

0.356

CXCL10/IP-10

ndc

IL-1β

31.3

1078

530

0.002

505

0.085

IL-6

29.8

2011

1048

0.069

1927

0.226

IL-1Ra

28.4

1.2

0.6

0.107

1.2

0.272

IL-1RI

22.6

0.9

0.5

< 0.001

0.8

0.301

TNF-α

ndc

iNOS

ndc

COX-2

26.58

133

48.3

0.11

186

0.03

LIF

23.8

35.2

19.2

0.003

28.9

0.239


IL-1β -stimulated cells were cultured with 31.3 - 1000 mg/L RHP or 1.2 - 38.7 mg/L of GLGPG for 4 h and gene expression was quantified by RT-PCR. Fold changes were calculated as indicated in Materials and Methods. Only the effects of 250 mg/L RHP and 9.7 mg/L GLGPG are shown.

aCT = cycle threshold (CT < 22 = high basal gene expression; 23 < CT < 30 = intermediate basal gene expression; CT > 30 = low basal gene expression).

bp value = significance value between (IL-1β)- and (IL-1β + substance) -treatment

cnd = not done

Schwager et al. BMC Complementary and Alternative Medicine 2011 11:105   doi:10.1186/1472-6882-11-105

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