Table 3

Effect of RHP and GLGPG on the production of chemokines and cytokines in human PBL

Protein

Ratioa

LPS/IFN-γ

LPS/IFN-γ + RHP (250 mg/L)

LPS/IFN-γ + GLGPG (9.7 mg/L)

pg/mL ± SD

pg/mL ± SD

p valueb

pg/mL ± SD

p valueb


Eotaxin

28

84 ± 2

62 ± 6

0.037

62 ± 3

0.013

MCP-1

497

2940 ± 226

4990 ± 71

0.007

1880 ± 368

0.074

MIP-1β

169

90900 ± 1838

109009 ± 1823

0.005

47550 ± 3465

0.004

MIP-1α

2092

21079 ± 1131

27510 ± 849

0.023

10950 ± 71

0.006

IL-8

794

325103 ± 12738

433040 ± 122728

0.059

267052 ± 36770

0.255

CCL5/RANTES

14

4470 ± 57

1011 ± 55

< 0.001

3210 ± 750

0.067

CXCL10/IP-10

47

12650 ± 16

150 ± 16

0.002

5025 ± 148

0.005

IL-1β

> 2000

21900 ± 2121

14350 ± 1344

0.051

9195 ± 106

0.014

IL-6

> 2000

79650 ± 4031

68450 ± 4031

0.083

38604 ± 2828

0.005

IL-12(p70)

> 200

295 ± 2

19 ± 2

0.002

69 ± 17

0.006

IL-10

67

233 ± 8

382 ± 3

0.002

143 ± 47

0.118

TNF-α

> 2000

21107 ± 990

6595 ± 290

0.003

5105 ± 1775

0.008

IFN-γ

672

2065 ± 106

226 ± 18

0.002

475 ± 2

0.002

GM-CSF

158

148 ± 5

190 ± 47

0.329

54 ± 2

0.002


LPS/IFN-γ -stimulated cells were cultured with 31.3 - 1000 mg/L RHP or 1.2 - 38.7 mg/L of GLGPG for 24 h and proteins were quantified by multiparametric analysis (see Materials and Methods). Only the effects of 250 mg/L RHP and 9.7 mg/L GLGPG are shown.

a protein secreted by stimulated/protein secreted by unstimulated cells

b p value = significance value between (LPS/IFN-γ)- and (LPS/IFN-γ + substance) -treatment.

Schwager et al. BMC Complementary and Alternative Medicine 2011 11:105   doi:10.1186/1472-6882-11-105

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