Table 2

Modulation of gene expression in RAW264.7 cells by RHP and GLGPG

Gene

CTa

LPS

LPS + RHP (250 mg/L)

LPS + GLGPG (9.7 mg/L)

fold change

fold change

p valueb

fold change

p valueb


TNF-α

22.9

29.6

21.8

0.0001

20.4

0.002

COX-2

27.5

247

203.3

0.347

217

0.660

PGES

33.0

2.2

1.6

0.0107

2.0

0.214

iNOS

29.9

197

239.4

0.041

224

0.357

IL-1α

33.7

22081

8128

0.0115

18501

0.087

IL-10

38.3

66.8

176

< 0.0001

78.6

0.074

CCL5/RANTES

29.7

515

121

< 0.0001

403

0.003

CXCL10/IP-10

27.2

342

65.4

0.0001

239

0.021

NF-κB1

24.8

6.3

2.1

0.0002

6.0

0.366

NF-κB49

25.3

4.1

1.5

0.0028

3.7

0.028

NF-κBp65

23.6

1.2

0.4

0.0045

1.1

0.126

I-κBα

24.4

5.5

2.0

0.0004

5.7

0.391

CD14

24.9

1.0

0.5

0.198

1.4

0.012

MMP-9

29.0

6.8

3.0

0.0493

7.8

0.145


LPS-stimulated cells were cultured with 31.3 - 1000 mg/L RHP or 1.2 - 38.7 mg/L of GLGPG for 4 h and gene expression was quantified by RT-PCR. Fold changes were calculated as indicated in Materials and Methods. Only the effects of 250 mg/L RHP and 9.7 mg/L GLGPG are shown.

aCT = cycle threshold (CT < 22 = high basal gene expression; 23 < CT < 30 = intermediate basal gene expression; CT > 30 = low basal gene expression).

bp value = significance value between (LPS)- and (LPS + compound)-treatment.

Schwager et al. BMC Complementary and Alternative Medicine 2011 11:105   doi:10.1186/1472-6882-11-105

Open Data