Open Access Research article

Effects of Hypericum Perforatum, in a rodent model of periodontitis

Irene Paterniti1*, Enrico Briguglio2*, Emanuela Mazzon3, Maria Galuppo1, Giacomo Oteri2, Giancarlo Cordasco1 and Salvatore Cuzzocrea12

Author Affiliations

1 Department of Clinical and Experimental Medicine and Pharmacology, School of Medicine, University of Messina, Italy

2 Istituto Policattedra di Odontostomatologia, University of Messina, Italy

3 IRCCS Centro Neurolesi "Bonino-Pulejo", Messina, Italy

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BMC Complementary and Alternative Medicine 2010, 10:73  doi:10.1186/1472-6882-10-73

Published: 23 November 2010



Hypericum perforatum is a medicinal plant species containing many polyphenolic compounds, namely flavonoids and phenolic acids. In this study we evaluate the effect of Hypericum perforatum in animal model of periodontitis.


Periodontitis was induced in adult male Sprague-Dawley rats by placing a nylon thread ligature around the lower 1st molars. Hypericum perforatum was administered at the dose of 2 mg/kg os, daily for eight days. At day 8, the gingivomucosal tissue encircling the mandibular first molar was removed.


Periodontitis in rats resulted in an inflammatory process characterized by edema, neutrophil infiltration and cytokine production that was followed by the recruitment of other inflammatory cells, production of a range of inflammatory mediators such as NF-κB and iNOS expression, the nitration of tyrosine residues and activation of the nuclear enzyme poly (ADP-ribose) polymerase; apoptosis and the degree of gingivomucosal tissues injury. We report here that Hypericum perforatum exerts potent anti-inflammatory effects significantly reducing all of the parameters of inflammation as described above.


Taken together, our results clearly demonstrate that treatment with Hypericum reduces the development of inflammation and tissue injury, events associated with periodontitis.