Table 2

Comparison of the antioxidant and free radical scavenging capacities of 70% methanolic crudes of Terminalia chebula, Terminalia belerica and Emblica officinalis

Name of Assay

70% methanolic crudes of

Standard

Values of Standard compounds


Terminalia chebula

Terminalia belerica

Emblica officinalis


TEAC Values

4.52 ± 0.12

1.01 ± 0.03

4.10 ± 0.17

--

--

† Phenolic content

127.60 ± 0.001

133.00 ± 0.003

215.60 ± 0.004

--

--

‡ Flavonoid content

219.30 ± 0.01

138.30 ± 0.01

176.00 ± 0.01

--

--

§ Ascorbic acid content

46.74 ± 1.10

45.25 ± 0.75

71.08 ± 1.63

--

--


♣ IC50 values of the extracts for free radical scavenging capacity for:


DPPH

1.73 ± 0.07***

1.45 ± 0.02***

1.43 ± 0.03***

Ascorbic acid

5.29 ± 0.28

Hydroxyl (OH.)

72.02 ± 8.99***

203.25 ± 1.87**

382.02 ± 7.88***

Mannitol

571.45 ± 20.12

Superoxide (O2.-)

13.42 ± 0.22***

18.18 ± 1.39***

13.82 ± 0.19***

Quercetin

42.06 ± 1.35

Nitric oxide (NO.)

33.28 ± 4.56***

40.83 ± 4.40***

33.89 ± 2.94***

Curcumin

90.82 ± 4.75

Peroxynitrite (ONOO.-)

1.27 ± 0.07***

0.99 ± 0.10**

0.83 ± 0.03NS

Gallic acid

0.88 ± 0.06

Singlet oxygen (1O2)

424.50 ± 24.70***

233.12 ± 48.68***

490.42 ± 159.59**

Lipoic acid

46.15 ± 1.16

Hypochlorous acid (HOCl)

433.60 ± 15.45**

271.51 ± 13.70**

420.58 ± 31.97***

Ascorbic acid

235.96 ± 5.75


† Phenolic content (mg/ml Gallic acid equivalent per 100 mg plant extract)

‡ Flavonoid content (mg/ml Quercetin equivalent per 100 mg plant extract)

§ Ascorbic acid content (mg/ml Ascorbic acid per 100 mg plant extract)

♣ IC50 Values are in μg/ml (except for Peroxynitrite scavenging assay where values are expressed in mg/ml). Each value represents mean ± S.D. (n = 6)

* p < 0.05; ** p < 0.01;*** p < 0.001; NS = Non significant

Hazra et al. BMC Complementary and Alternative Medicine 2010 10:20   doi:10.1186/1472-6882-10-20

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