Table 3

Background information concerning the use of the BacLight® assay (BLA) for assessment of (dental) biofilm vitality
Studies using the BLA (n = 30) [5-7,9,16-20,22,23,41,71,73],[82]* [83,85-98]
(a) Were plaque-like biofilms evaluated? No: [5,16-18,22,23,41,82,83,86],[87,93,96-98]
Yes: [6,7,9,19,20,71,73,85],[88-92,94,95]
(b) Validation No: [5,7,9,16-18,20,22,23,41],[71,73,82,85-91,94-98]
Yes: [6, 19?, 83, 92, 93?]
(c) Dilution factor Not stated: [16,18,23,73,86,94,95,98]
1:1 [6,7,9,17,20,22,41,71],[85,87-92,96,97]
2:1 [93]
4:1 [5]
6:4 [19]
1:6 [83]
(d) Incubation procedure Not stated: [16,18,71,86,95,96]
10 min, RT [6,7,20]
15 min, RT [5,9,17,19,22,23,41,73],[85,87-91,97,98]
20 min, RT [83,92]
>20 min, 2°C [93]
30 min [94]
(e) Comparison No: [16-18,20,22,71,82,83,85,88],[90,91,94,95]
Yes: [5-7,9,19,23,41,73,86,87],[89,92,93,96-98]
(f) … with inappropriate methods [6,7,9,19,23,41,73,86],[87,89,92,93,96-98]
(g) … with appropriate methods [5]*
(h) Did the BLA results fit to the other parameters? No: [7,19,86,89,92,93] (DAPI), [96,97]
Yes: [5,23,41,93] (FDA), [98]
(i) Did the BLA results meet the expectations of the user(s)? No: [23,83,86]
Yes: [5,7,16,18-20,22,41,71,82],[85,87-98]

(a) to (i) see description in the text.

Not stated: Either no information was given by the authors, or the authors stated that the staining was conducted “according to the manufacturer’s instructions”, what means generally a dilution factor of 1:1, and an incubation time of 15 minutes.

*[5]: Decker registered the total bacterial counts (as log BC/ml) and the CFU (as log CFU/ml), however gave no data regarding the PE.

*[82]: “cell-free” physicochemical measurements to elucidate the mechanism of the BacLight staining procedure.

Netuschil et al.

Netuschil et al. BMC Oral Health 2014 14:2   doi:10.1186/1472-6831-14-2

Open Data