|
Differential chemical shifts between pairs of hybrid, Cp and Pf A2K rubredoxins. |
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| 1HN |
15N |
1H(all) |
1HN(con)a |
15N(con) |
1H(con) |
|
|
|
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| Pf metal site-swapped Cp hybrid-parentb |
0.046 |
0.279 |
0.042 |
0.049 |
0.318 |
0.043 |
| Pf metal site-swapped Cp hybrid-non-parentc |
- |
- |
- |
0.301 |
1.872 |
0.191 |
| Cp metal site-swapped Pf hybrid-parent |
0.044 |
0.264 |
0.038 |
0.049 |
0.277 |
0.042 |
| Cp metal site-swapped Pf hybrid-non-parent |
- |
- |
- |
0.334 |
1.871 |
0.175 |
| Cp - Pf A2K |
- |
- |
- |
0.286 |
1.785 |
0.181 |
|
a. Resonances from residues that are conserved between the parental sequences. b. Residue-specific parental type assignment based on minimal amide chemical shift difference. c. Cp or Pf A2K resonance not assigned to the hybrid resonance based on amide chemical shift. | ||||||
LeMaster et al. BMC Structural Biology 2007 7:81 doi:10.1186/1472-6807-7-81 |
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