Figure 2.

Correlations among all hybrid and parental rubredoxin ¹H chemical shifts. The amide chemical shift data of Figure 1 were used to assign each individual residue of the metal binding site-swapped hybrids to a particular parent. Panel A illustrates the comparison to parental chemical shifts for the metal binding site-swapped Cp rubredoxin, while the analogous ¹H data for the complementary metal binding site-swapped Pf A2K rubredoxin hybrid are shown in panel B. Only the H^ε resonances of Lys 46 (open diamonds) deviate substantially from equivalence.