Table 8

Mutated and mutant residues not in secondary structuresa

Amino acid group

Original residues

Expected residues

χ2

P value

Mutant residues

Expected residues

χ2

P value


A

18

22

0.84

3.58E-01

21

29

2.16

1.42E-01

C

12

7

4.51

3.37E-02

25

17

3.93*

4.73E-02

D

46

34

4.20

4.04E-02

17

19

0.27

6.06E-01

E

10

22

6.53**

1.06E-02

17

17

0.00

9.72E-01

F

15

17

0.17

6.83E-01

13

19

2.04

1.53E-01

G

35

32

0.37

5.40E-01

26

28

0.10

7.48E-01

H

27

14

13.05***

3.04E-04

24

19

1.16

2.81E-01

I

19

17

0.23

6.32E-01

16

25

3.41

6.48E-02

K

11

27

9.15**

2.49E-03

13

17

0.88

3.47E-01

L

29

36

1.36

2.44E-01

43

40

0.27

6.04E-01

M

6

9

0.93

3.36E-01

13

11

0.43

5.11E-01

N

10

23

6.96**

8.34E-03

30

19

5.98**

1.45E-02

P

61

52

1.44

2.29E-01

26

29

0.29

5.90E-01

Q

3

20

13.97***

1.86E-04

17

17

0.00

9.72E-01

R

53

23

37.42***

9.51E-10

49

41

1.59

2.08E-01

S

31

40

2.13

1.45E-01

47

45

0.13

7.14E-01

T

24

30

1.25

2.63E-01

22

29

1.65

1.99E-01

V

25

25

0.00

9.44E-01

25

29

0.53

4.68E-01

W

8

6

0.41

5.22E-01

11

8

0.78

3.76E-01

Y

29

17

8.77**

3.06E-03

17

14

0.45

5.02E-01


Sum

472

472

472

472


aχ2-numbers in italics indicate underrepresentation and numbers in bold overrepresentation compared to random distribution based on amino acid frequencies. The results of the χ2 are shown with significance level: * P < 0.05; ** P < 0.01; *** P < 0.001.

Khan and Vihinen BMC Structural Biology 2007 7:56   doi:10.1186/1472-6807-7-56

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