Table 6

Spectrum of mutations in β-strandsa

Amino acid

Original residues

Expected residues

χ2

P value

Mutant residues

Expected residues

χ2

P value


A

39

33

0.93

3.34E-01

14

34

11.49***

6.98E-04

C

19

12

4.58*

3.24E-02

39

20

19.08***

1.26E-05

D

17

15

0.22

6.38E-01

26

22

0.56

4.54E-01

E

21

25

0.64

4.23E-01

20

20

0.01

9.36E-01

F

15

36

12.66***

3.75E-04

24

22

0.11

7.43E-01

G

44

27

10.61***

1.13E-03

27

32

0.86

3.54E-01

H

23

13

8.33**

3.90E-03

32

22

4.06*

4.38E-02

I

34

49

4.80*

2.84E-02

16

29

6.15**

1.31E-02

K

13

23

4.60*

3.20E-02

23

20

0.57

4.49E-01

L

63

66

0.10

7.48E-01

27

46

8.05**

4.56E-03

M

14

13

0.03

8.53E-01

16

13

0.90

3.43E-01

N

19

14

1.67

1.97E-01

20

22

0.27

6.05E-01

P

15

11

1.08

2.99E-01

47

34

5.27*

2.16E-02

Q

10

16

2.24

1.34E-01

20

20

0.01

9.36E-01

R

77

29

80.03***

3.69E-19

60

48

3.17

7.50E-02

S

15

23

2.88

8.98E-02

55

52

0.18

6.68E-01

T

16

35

10.20***

1.41E-03

36

34

0.16

6.88E-01

V

53

71

4.44*

3.51E-02

27

34

1.32

2.50E-01

W

16

10

3.23

7.25E-02

8

10

0.34

5.61E-01

Y

27

27

0.00

9.60E-01

13

17

0.87

3.50E-01


Sum

550

550

550

550


aχ2-numbers in italics indicate underrepresentation and numbers in bold overrepresentation compared to random distribution based on amino acid frequencies. The results of the χ2 are shown with significance level: * P < 0.05; ** P < 0.01; *** P < 0.001.

Khan and Vihinen BMC Structural Biology 2007 7:56   doi:10.1186/1472-6807-7-56

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