Figure 1.

Multiple sequence alignment of R.Eco29kI and related REases with the experimentally solved structure of the catalytic domain of I-TevI homing nuclease used as a modeling template. The predicted secondary structure of R.Eco29kI is shown above the alignment, the structure of I-TevI is shown below the alignment (H, helix; e, extended). Identical residues are shown in black, the other conserved residues are in grey. Amino acid residues analyzed in this work are labeled.