|
Thermodynamic parameters of the binding between p41 and the WT and R21A variants of the Spc-SH3 domain at 25°C measured by ITC1 |
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| pH |
Protein |
Kb (×10-3 M-1) |
ΔHb (kJ mol-1) |
p |
TΔSb (kJ mol-1) |
ΔGb (kJ mol-1) |
|
|
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| 7.0 |
R21A |
18.1 ± 1.9 |
-58.7 ± 2.5 |
0.06 ± 0.05 |
-34.4 |
-24.3 |
| WT |
5.2 ± 1.2 |
-45.4 ± 6.3 |
0.01 ± 0.12 |
-24.2 |
-21.2 |
|
| Abl-SH3 |
323 |
-91.6 |
0.1 |
-60.2 |
-31.4 |
|
|
|
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| 3.0 |
R21A Spc-SH3 |
18.2 ± 2.0 |
-51.3 ± 1.7 |
-0.29 ± 0.08 |
-27.0 |
-24.3 |
| WT Spc-SH3 |
5.8 ± 0.8 |
-43.9 ± 3.3 |
-0.07 ± 0.11 |
-22.4 |
-21.5 |
|
| Abl-SH3 |
210 |
-93.3 |
- |
-62.9 |
-30.4 |
|
|
1The similar parameters for the Abl-SH3 domain are also shown for comparison and are taken from reference 16. The errors in each parameter were estimated from 95% confidence intervals as determined from the fittings of the binding isotherms. | ||||||
Casares et al. BMC Structural Biology 2007 7:22 doi:10.1186/1472-6807-7-22 |
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