Figure 2.

Multiple sequence alignment of Rossmann-fold methyltransferases and nicotianamine synthase. Sequences are denoted by NCBI gi number, short protein name (when available, otherwise COG/KOG/Pfam number was used), and abbreviated species name (as in UniProt Knowledgebase [140]). Nicotianamine synthase is marked by a blue box. Conserved motifs are labeled above the alignment. Conserved residues are marked by asterisk. Consensus positions of the secondary structure elements are shown above the alignment. Numbers in parentheses indicate number of residues omitted for clarity. Residues are highlighted according to the amino acid properties. Gray shading indicates conservation of single residue. Red font indicates conservation of acidic residues (D, E). Cyan font indicates conservation of Ser/Thr (S, T). Blue gray font with yellow shading indicates conservation of aliphatic residues (I, L, V). Dark blue font indicates conservation of basic residues (H, K, and R). Green font indicates conservation of tiny residues (A, G, and S). Blue font with yellow shading indicates conservation of aromatic residues (F, H, W, and Y). Pink font indicates conservation of charged residues (D, E, H, K, and R). Dark green font indicates conservation of small residues (A, C, D, G, N, P, S, T, and V). Bright blue font indicates conservation of polar residues (C, D, E, H, K, N, Q, R, S, and T). Blue font with pale yellow shading indicates conservation of big residues (E, F, H, I, K, L, M, Q, R, W, and Y). Black font with yellow shading indicates conservation of hydrophobic residues (A, C, F, G, H, I, L, M, T, V, W, and Y).

Kozbial and Mushegian BMC Structural Biology 2005 5:19   doi:10.1186/1472-6807-5-19
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