Tyk2 is protected from proteolysis by the addition of Compound 2. Shown are the combined, quantitated values of Tyk2 peaks of ~27 and ~29 kDa during digestion with thermolysin, in the absence or presence of 30 μM Compound 2. Intact Tyk2 protein runs as ~29 kDa. The ~27 kDa form produced by thermolysin digestion is unaffected by the presence of Compound 2.
Argiriadi et al. BMC Structural Biology 2012 12:22 doi:10.1186/1472-6807-12-22