Table 4

Angles and distances calculated for ALG-2 EF3. ALG-2 EF3 has α4 (entering helix, residue No. 93-102) and α5 (exiting helix, residue No. 112-122), but a segment of α5 (residue No. 112-120, common to ALG-2^ΔGF122) was used.

Angle (°)

Distance (Å)

θ

φ

ω

inner ends

midpoint

outer ends

WT

MF^a

49.5

104

137

10.6

12.2

14.7

Ca^b

57.6

96.9

131

10.7

12.7

15.6

Zn^c

54.5

98.0

130

10.7

12.6

15.4

ΔGF122

Ca^d

58.6

88.8

134

10.7

13.1

16.3

F122A

Zn^e

62.6

97.6

128

11.0

13.2

16.4

^a Metal-free form of des3-20ALG-2

^b Ca²⁺-bound form of des3-20ALG-2

^c Zn²⁺-bound form of ALG-2

^d Ca²⁺-bound form of des3-23ALG-2^ΔGF122

^e Zn²⁺-bound form of des3-20ALG-2^F122A

Angles and distances were estimated by the Vector Geometry Mapping (VGM) software (Ref. 50)

Inuzuka et al. BMC Structural Biology 2010 10:25   doi:10.1186/1472-6807-10-25