Bridging the phenotypic gap: Real-time assessment of mitochondrial function and metabolism of the nematode Caenorhabditis elegans

Additional file 1:

Time course of strain PE255 luminescence following addition of luciferin (at t = 0). Luminescence buffer, consisting of citrate phosphate buffer pH 6.5, 0.1 mM D-luciferin, 1% DMSO and 0.05 % triton-X (all final concentrations) or 0.1 mM D-luciferin (final concentration) in citrate phosphate buffer pH 6.5 (without DMSO or triton-X) was added to wells containing 15 unsynchronised gravid worms. Luminescence was measured in a Clarity luminometer using the KC4 programme. Luminescence increased rapidly after adding luciferin, reaching its maximum levels within the second min, but remaining relatively stable for the first 5 min, followed by a gradual decrease in luminescence. The presence of 1% DMSO and 0.05 % triton-X increases luminescence by 2 to 2.5 times.

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