Research article

Functional and pharmacological characterization of a Shal-related K⁺ channel subunit in Zebrafish

Tomoe Y Nakamura¹ and William A Coetzee²

¹Department of Molecular Physiology, National Cardiovascular Center Research Institute, Osaka, Japan

²Departments of Pediatrics, Pharmacology and Physiology & Neurosciences NYU School of Medicine, New York, NY, USA

author email corresponding author email

BMC Physiology 2008, 8:2doi:10.1186/1472-6793-8-2

Published:	8 February 2008

Abstract

Background

K⁺ channels are diverse; both in terms of their function and their molecular composition. Shal subunits were first described in Drosophila. There are three mammalian orthologs, which are members of the Kv4 subfamily. They are involved in neuronal firing patterns as well as control of the cardiac action potential duration.

Results

Here, we report the biophysical and pharmacological characterization of zShal3, which is the ortholog of the mammalian Kv4.3 subunit, which in mammals is involved in action potential repolarization and gives rise to neuronal A-type K⁺ currents involved in somatodendretic signal integration.

Conclusion

We demonstrate that zShal has similar functional and pharmacological characteristics compared to Kv4.3 and it is similarly regulated by pharmacological agents and by the Kv4 accessory subunit, NCS-1.