Epigenetic and phenotypic changes result from a continuous pre and post natal dietary exposure to phytoestrogens in an experimental population of mice

doi:10.1186/1472-6793-8-17

BMC Physiology

Volume 8

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Valladares LE
Clark SJ

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Research article

Epigenetic and phenotypic changes result from a continuous pre and post natal dietary exposure to phytoestrogens in an experimental population of mice

Carlos M Guerrero-Bosagna¹^,2^,4^,5 , Pablo Sabat²^,3 , Fernanda S Valdovinos² , Luis E Valladares⁴ and Susan J Clark⁵

¹Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, WA, 99164-4231, USA

²Laboratorio de Ecofisiología Animal, Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, Chile

³Center for Advanced Studies in Ecology & Biodiversity and Departamento de Ecología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica, de Chile, Santiago, Chile

⁴Laboratorio de Hormonas y Receptores, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Santiago, Chile

⁵Epigenetics Laboratory, Cancer Program, Garvan Institute of Medical Research, Sydney, Australia

author email corresponding author email

BMC Physiology 2008, 8:17doi:10.1186/1472-6793-8-17


Published:	15 September 2008

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Additional file 1:

Comparison of methylation in ERα promoter in liver. Methylation in the same samples was compared with two different procedures after bisulphite conversion: direct sequencing measured by raw data versus cloning and sequencing. This comparison was randomly performed in three samples in order to verify the reproducibility of direct sequencing measured by raw data with regard to cloning and sequencing. Left figures show cloning results and right plots show the comparison with direct sequencing measured by raw data. Methylated CpG sites are indicated as black circles (●) and unmethylated CpG sites as white circles (○). The sample number representing each animal is shown in the figure: (a) animal 1 (male, control); (b) animal 2 (female, control); and (c) animal 23 (female, ISF).

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