Research article
Epigenetic and phenotypic changes result from a continuous pre and post natal dietary exposure to phytoestrogens in an experimental population of mice
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* Corresponding author: Carlos M Guerrero-Bosagna guerrerobosagna@gmail.com
1 Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, WA, 99164-4231, USA
2 Laboratorio de Ecofisiología Animal, Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, Chile
3 Center for Advanced Studies in Ecology & Biodiversity and Departamento de Ecología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica, de Chile, Santiago, Chile
4 Laboratorio de Hormonas y Receptores, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Santiago, Chile
5 Epigenetics Laboratory, Cancer Program, Garvan Institute of Medical Research, Sydney, Australia
BMC Physiology 2008, 8:17 doi:10.1186/1472-6793-8-17
Published: 15 September 2008Additional files
Additional file 1:
Comparison of methylation in ERα promoter in liver. Methylation in the same samples was compared with two different procedures after bisulphite conversion: direct sequencing measured by raw data versus cloning and sequencing. This comparison was randomly performed in three samples in order to verify the reproducibility of direct sequencing measured by raw data with regard to cloning and sequencing. Left figures show cloning results and right plots show the comparison with direct sequencing measured by raw data. Methylated CpG sites are indicated as black circles (●) and unmethylated CpG sites as white circles (○). The sample number representing each animal is shown in the figure: (a) animal 1 (male, control); (b) animal 2 (female, control); and (c) animal 23 (female, ISF).
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