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List of bioassays used for characterization in the initial screen and result summary |
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| Therapeutic Field |
Bioassays |
Phenotype |
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| Cardiovascular, renal and bladder function |
Bladder function (F) |
NS |
| Blood pressure/heart rate (M) |
↑(BP)* |
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| Urine chemistry panel (F) |
NS |
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| CNS and Behavior |
Irwin test (M,F) |
↓*** |
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| Cross-therapeutic assays |
Automated blood chemistry panel (M) |
NS |
| Body weight measurements (M,F) |
NS |
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| Corticosterone (M) |
↑* |
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| Immunology and Inflammation |
DTH (M) |
NS |
| FACS analysis (M) |
↓* |
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| LPS challenge (M) |
NS |
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| Monocyte infiltration (F) |
↑* |
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| Pulmonary inflammation (F) |
NS |
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| Wound healing (F) |
NS |
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| Metabolism and Body Composition |
DEXA (F) |
↓* |
| Excised bone DEXA (F) |
↓** |
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| Food intake (M,F) |
NS |
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| Food intake on HFD (M) |
↑*** |
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| Leptin (M,F) |
↓** |
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| Metabolic response to HFD (M) |
↑** |
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| Physical response to HFD (M) |
↓*** |
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| Selected muscle weight (F) |
↓** |
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| Pain |
Formalin pain assay (M) |
NS |
| Hot-plate assay (F) |
NS |
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| Sexual health |
Histopathology of reproductive system (M) |
NS |
| Induced erection test (M) |
↓* |
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| Male contact sexual behavior (M) |
↓*** |
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| Male fertility (M) |
↓*** |
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| Testosterone (M) |
NS |
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The highest levels of significance among all measures measured in the assay are shown by asterisks: * P < 0.05, ** 0.001 < P < 0.01, *** P < 0.001 and arrows indicate measurements in the TG line increased or decreased from WT. No significant difference between genotypes (NS), males (M), females (F), delayed-type hypersensitivity (DTH), fluorescence-activated cell sorting (FACS), lipopolysaccharide (LPS), Dual energy X-ray analysis (DEXA) high fat diet (HFD). | ||
Hayward et al. BMC Physiology 2007 7:13 doi:10.1186/1472-6793-7-13 |
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