Open Access Highly Accessed Research article

The inflammatory and normal transcriptome of mouse bladder detrusor and mucosa

Marcia R Saban1, Helen L Hellmich2, Mary Turner3, Ngoc-Bich Nguyen17, Rajanikanth Vadigepalli4, David W Dyer5, Robert E Hurst6, Michael Centola3 and Ricardo Saban1*

Author Affiliations

1 Department of Physiology, The University Oklahoma Health Sciences Center, Oklahoma City, USA

2 Department of Anesthesiology, University of Texas Medical Branch, Galveston, USA

3 Oklahoma Medical Research Foundation (OMRF), Arthritis and Immunology Research Program, Microarray Core Facility, Oklahoma City, USA

4 Daniel Baugh Institute for Functional Genomics and Computational Biology. Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, USA

5 Department of Microbiology and Immunology, Laboratory for Genomics and Bioinformatics, Oklahoma University Health Sciences Center, Oklahoma City, USA

6 Department of Urology, The University Oklahoma Health Sciences Center, Oklahoma City, USA

7 Cellular & Structural Biology, The University of Texas Health Science Center at San Antonio, San Antonio, USA

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BMC Physiology 2006, 6:1  doi:10.1186/1472-6793-6-1

Published: 18 January 2006

Additional files

Additional File 1:

Separation of bladder layers (mucosa and detrusor).

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Additional File 2:

Detailed methodology for SSH and analysis of the experimental procedures.

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Additional File 3:

Analysis of ligation.

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Additional File 4:

Analysis of PCR products.

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Additional File 5:

The reduced message of a known housekeeping gene between the subtracted and un-subtracted cDNA over the same number of PCR cycles.

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