Figure 4.

Co-immunostaining of AQP8 and AE2 by confocal immunofluorescence. WIF-B cells were fixed, permeabilized, and labelled simultaneously with rabbit anti-AE2 and goat anti-AQP8. Fluorescence localization was viewed by laser scanning confocal microscopy (see "Materials and Methods" for details). *, bile canaliculi structures.