Table 2

RT-PCR demonstration of the presence of WNK1 and absence of WNK4 in rat SCCD primary cultures
Tissue 18S WNK1 WNK4
rSCCD 13.4 24.7 31.6
rat kidney 14.7 25.0 26.0
blank 34.4  BT 35.2

Ct, average. BT, Below Threshold fluorescence at end of 38 thermocycles. Ct values are based on duplicate runs of 4 samples SCCD and duplicates of rat kidney as a control tissue. The difference between Ct values of duplicates varied by 0.6 ± 0.09 (n=36) with a range from 0.0 to 2.1.

Pondugula et al.

Pondugula et al. BMC Physiology 2013 13:6   doi:10.1186/1472-6793-13-6

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