Inhibition of forskolin-stimulated Iscby bumetanide and DIOA. A) Summary of inhibition by basolateral bumetanide (BUM) (n = 11) of cAMP-stimulated Isc (forskolin, FSK + IBMX). Epithelia were incubated in dexamethasone and Na+ transport was inhibited with apical amiloride before stimulation by cAMP. Note that Isc does not return after only bumetanide to the basal Cl- secretion rate in amiloride (dotted line). B) Representative recording of VT and the response to apical amiloride forskolin + IBMX, basolateral DIOA and basolateral bumetanide (BUM). C) Summary of response of Isc to DIOA and bumetanide (BUM) (n = 3) after stimulation by forskolin+ IBMX. Na+ transport was inhibited with apical amiloride before stimulation. Note that Isc returns after both DIOA and bumetanide to the basal Cl- secretion rate in amiloride (dotted line). Summary data (A and C) are mean ± SEM; *, P < 0.05 compared to bar immediately to the left; NSA, not significantly different from amiloride.
Pondugula et al. BMC Physiology 2013 13:6 doi:10.1186/1472-6793-13-6