Open Access Research article

Regional expression of HOXA4 along the aorta and its potential role in human abdominal aortic aneurysms

John H Lillvis1, Robert Erdman2, Charles M Schworer2, Alicia Golden2, Kimberly Derr2, Zoran Gatalica3, Laura A Cox45, Jianbin Shen16, Richard S Vander Heide7, Guy M Lenk1, Leigh Hlavaty8, Li Li16, James R Elmore9, David P Franklin9, John L Gray9, Robert P Garvin9, David J Carey2, Wayne D Lancaster110, Gerard Tromp2 and Helena Kuivaniemi2*

Author Affiliations

1 Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, Michigan, USA

2 Sigfried and Janet Weis Center for Research, Geisinger Clinic, Danville, 100 North Academy Avenue, Pennsylvania 17822-2610, USA

3 Department of Pathology, Creighton University School of Medicine, Omaha, Nebraska, USA

4 Southwest National Primate Research Center, San Antonio, Texas, USA

5 Department of Genetics, Texas Biomedical Research Institute, San Antonio, Texas, USA

6 Department of Internal Medicine, Wayne State University School of Medicine, Detroit, Michigan, USA

7 Department of Pathology, Louisiana Health Sciences Center, New Orleans, Louisiana, USA

8 Office of Wayne County Medical Examiner, Detroit, Michigan, USA

9 Department of Vascular and Endovascular Surgery, Geisinger Clinic, Danville, Pennsylvania, USA

10 Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, Michigan, USA

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BMC Physiology 2011, 11:9  doi:10.1186/1472-6793-11-9

Published: 31 May 2011

Additional files

Additional file 1:

Table S1. Human tissue samples from non-aneurysmal aortas used in assessing protein abundance of HOXA4. List of sample IDs with donor age, ethnicity, sex and cause of death for human samples used to study HOXA4 protein levels.

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Additional file 2:

Table S2. Human aortic tissue samples used in microarray and Q-RT-PCR experiments. List of sample IDs with donor disease status classification, ethnicity, sex, age, and cause of death for human samples used in microarray and RT-PCR studies.

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Additional file 3:

Figure S1. Specificity of HOXA4, ACTA2 and ACTB antibodies. Images of western blots performed with antibodies used in the study.

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Additional file 4:

Table S3. Commercially available human cell lines for HOXA4 experiments. List of cell lines used in the study with codes, description of cell type, vendor, and catalog number.

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Additional file 5:

Table S4. mRNA expression of HOX genes in human aneurysmal (N = 6) and non-aneurysmal (N = 7) abdominal aorta based on microarray analyses. List of differentially expressed homeodomain-containing genes in human AAA compared to age-sex-and ethnicity-matched controls. Columns give the full protein name, Gene Symbol, GeneID, expression levels in control group and AAA group, fold-change in expression, False Discovery Rate and Illumina Detection Score in control and AAA group.

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Additional file 6:

Figure S2. Localization of HOXA4 protein in cultured human aortic ECs (A) and SMCs (B) using immunofluorescence. Images of stained cultured cells.

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