P1NP levels after treatments with gut hormones in three osteoblastic cell lines. Cells were serum deprived and treated for 48 or 120 h with the gut peptides at indicated molar concentrations. Straight lines solid square markers designate 48 h treatment; dashed lines emptied square markers indicate 120 h treatment. Significant higher production of P1NP after 120 h exposure to GIP and OB were observed only in Saos-2 (C, O). The least mature cell lines, MG-63 and TE-85 showed decreased P1NP secretion in response to GIP (A), GLP-1 (D, E), GLP-2 (H) and OB (N) stimulation after 120 h exposure. In all the cases the most significant changes were observed with the lowest concentration of peptide. Results are the average of percentage of change in relation to controls; n = 5. ± SEM.
Pacheco-Pantoja et al. BMC Physiology 2011 11:12 doi:10.1186/1472-6793-11-12