Figure 4.

ALP levels after treatments with gut hormones in three osteoblastic cell lines. Cells were treated for 48 or 120 h with modified medium containing the gut peptides at indicated molar concentrations. Straight lines circle solid markers designate 48 h treatment; dashed lines emptied circle markers indicate 120 h treatment. Only Saos-2 and TE-85 displayed statistically significant responses to gut peptides in ALP production. Saos-2 responded to GIP (C), GHR (L) and OB (O). TE-85 was stimulated only by GHR (K). MG-63 did not show any significant changes. Results are the average of percentage of change in relation to controls; n = 10, ± SEM.