Cell viability levels after treatments with gut hormones in three osteoblastic cell lines. Cells were treated for 48 or 120 h with the gut peptides at indicated molar concentrations. Straight lines solid diamond markers designate 48 h treatment; dashed lines emptied diamond markers indicate 120 h treatment. In this assay, GIP induced statistically significant increases in Saos-2 after 120 h in a dose-dependent manner (C), GLP-1 (D,E), GLP-2 (G, H)) and GHR (J, K) caused increases in MG-63 and TE-85, and the only positive responses for OB were observed in TE-85 (N). Results are the average of percentage of change in relation to controls; n = 10. ± SEM.
Pacheco-Pantoja et al. BMC Physiology 2011 11:12 doi:10.1186/1472-6793-11-12