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Open Access Highly Accessed Open Badges Research article

The actions of exogenous leucine on mTOR signalling and amino acid transporters in human myotubes

Petra Gran and David Cameron-Smith*

Author Affiliations

Molecular Nutrition Unit, School of Exercise and Nutrition Sciences, Deakin University, Burwood, Victoria, Australia

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BMC Physiology 2011, 11:10  doi:10.1186/1472-6793-11-10

Published: 25 June 2011

Additional files

Additional file 1:

Protein abundance of total mTOR is not affected by either leucine or insulin. A figure representing the protein expression of total mTOR at 30 min, 3 h and 24 h. Primary human myotubes were untreated or incubated in the presence of leucine (Leu; 5 mM), insulin (Ins; 100 nM) or co-treated with leucine and insulin (Leu + Ins) for 30 min (A), 3 h (B) or 24 h (C). Protein abundance of total mTOR was determined by Western blot analysis. Data are presented as mean ± SEM (n = 6).

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