The actions of exogenous leucine on mTOR signalling and amino acid transporters in human myotubes
Molecular Nutrition Unit, School of Exercise and Nutrition Sciences, Deakin University, Burwood, Victoria, Australia
BMC Physiology 2011, 11:10 doi:10.1186/1472-6793-11-10Published: 25 June 2011
Additional file 1:
Protein abundance of total mTOR is not affected by either leucine or insulin. A figure representing the protein expression of total mTOR at 30 min, 3 h and 24 h. Primary human myotubes were untreated or incubated in the presence of leucine (Leu; 5 mM), insulin (Ins; 100 nM) or co-treated with leucine and insulin (Leu + Ins) for 30 min (A), 3 h (B) or 24 h (C). Protein abundance of total mTOR was determined by Western blot analysis. Data are presented as mean ± SEM (n = 6).
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