Figure 6.

Effects of marathon on SIRT1, SIRT3 and SIRT4 in mRNA expression. SIRT1, SIRT3 and SIRT4 RNA levels were estimated by Northern Blot. RNA was extracted, electrophoresed and hybridized with a labelled probe as described under Methods β-actin was used as loading control. Blots are representative of at least three separate experiments. (P < 0.0001)