Table 2

proteases of D. magna

Protease

Accession

number

Primer forward

Primer reverse

Apparent

MW [kDa]

True

MW [kDa]


T152

DMC00580

5'-TGGATGCTCCATTGGACTTGA-3'

5'-CGGAAACGGTGACGATGATTC-3'

32-34

25.3

T208

DMC05983

5'-TGCGTTAGAGGAGTTGACGCT-3'

5'-TGAAGCTGACAACACCACGGT-3'

24-25

CT383

DMC00886

5'-TTGGCACCTTCCACCGAAT-3'

5'-TCATCAGGACTGGAGAAACGC-3'

20

23.98

CT448

DMC01045

5'-TCATCAACGGAGCTGAGGCTA-3'

5'-AGAACCCACTGGTCGGAAATC-3'

21-22

26.4

T610

DMC00622

75

24.1

CT802

DMC00275

5'-TCAGATTGCCCAACCCCAT-3'

5'-TCCGCTGATGTGGAGCATATC-3'

17-19

24.7


Delineated are the names and the accession numbers of the proteases of D. magna from the EST-database search and the established forward and reverse primers for the QPCR analyses (no primers for T610 were established). Apparent molecular weights (MW) resulted from the comparison of the molecular weight marker with the visible protease bands in the SDS-PAGE, whereas true MW resulted from the translation of the sequences (start- to stop-codon) of the EST-database (http://www.nematodes.org/NeglectedGenomes/ARTHROPODA/Crustacea.html webcite; [34]). No complete sequence was available for T208. (CT = chymotrypsin, T = trypsin).

Schwarzenberger et al. BMC Physiology 2010 10:6   doi:10.1186/1472-6793-10-6

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