SDS-PAGE of homogenate of D. magna grown on different food treatments. Activity stained SDS-PAGE of homogenate of D. magna grown on either 100% S. obliquus, 20% M. aeruginosa NIVA Cya 43 (with chymotrypsin inhibitors) and 80% S. obliquus, or on 20% M. aeruginosa PCC 7806 Mut (with trypsin inhibitors) and 80% S. obliquus. White bands indicate active proteases. Arrows point at shifts in the protease pattern. The numbers to the right depict the results of LC-MS/MS analysis and database search of the proteases (CT = chymotrypsin, T = trypsin).
Schwarzenberger et al. BMC Physiology 2010 10:6 doi:10.1186/1472-6793-10-6