Open Access Highly Accessed Research article

Diet-induced obesity in zebrafish shares common pathophysiological pathways with mammalian obesity

Takehiko Oka1, Yuhei Nishimura1234, Liqing Zang5, Minoru Hirano1, Yasuhito Shimada1234, Zhipeng Wang1, Noriko Umemoto1, Junya Kuroyanagi1, Norihiro Nishimura45 and Toshio Tanaka1234*

Author Affiliations

1 Department of Molecular and Cellular Pharmacology, Pharmacogenomics and Pharmacoinformatics, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507, Japan

2 Department of Bioinformatics, Mie University Life Science Research Center, 2-174 Edobashi, Tsu, Mie 514-8507, Japan

3 Department of Medical Chemogenomics, Mie University Venture Business Laboratory, 2-174 Edobashi, Tsu, Mie 514-8507, Japan

4 Mie University Medical Zebrafish Research Center, 2-174 Edobashi, Tsu, Mie 514-8507, Japan

5 Department of Translational Medical Science, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507, Japan

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BMC Physiology 2010, 10:21  doi:10.1186/1472-6793-10-21

Published: 21 October 2010

Additional files

Additional file 1:

Supplemental Table S1. Genes dysregulated in DIO zebrafish (OF8W vs OF1W)

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Additional file 2:

Supplemental Table S2. Genes ameliorated by CR in zebrafish DIO (CR2W vs OF8W)

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Additional file 3:

Supplemental Table S3. Genes dysregulated in DIO rats (GSE8700)

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Additional file 4:

Supplemental Table S4. Genes dysregulated in DIO mice (GSE11790)

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Additional file 5:

Supplemental Table S5. Genes dysregulated in obese humans (GSE15524)

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Additional file 6:

Supplemental Table S6. Genes differentially expressed in visceral AT in the same direction in at least two obesity models

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Additional file 7:

Supplemental Figure S1. Validation of the differential gene expression by qPCR analysis. Total RNA was extracted from zebrafish visceral AT and qPCR analysis was performed to validate the differential expression identified by microarray analysis. Results represent means ± S.E.M. of each group. P-value was calculated by Student's t-test using OF1W (N = 6) vs. OF8W (N = 4). *p < 0.05.

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Additional file 8:

Supplemental Table S7. Primers used in qPCR analysis

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