Table 1 |
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|
PCR primers, degeneracy and annealing temperatures (Ta). |
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|
Primer name |
Primer sequence (5'-3')1 |
Degeneracy |
Ta°C |
|
|
|||
|
UTX/UTY |
TGCTACCTCAGGTGGACAACAAGG |
1 |
62 |
|
UTY |
TGCTTGTTTCAGGCACCAAGGRTCTATK |
4 |
62 |
|
UTX |
CTCGACACTGGCAGTGCTGTTAGG |
1 |
62 |
|
|
|||
|
1Degenerate nucleotides are shown in bold. |
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|
Villesen and Fredsted BMC Ecology 2006 6:8 doi:10.1186/1472-6785-6-8 |
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