Table 3

Feature overview of different image analysis software. Comparison of 2 recent published image analysis programs COMSTAT and ISA-3D with PHLIP. Feature information of COMSTAT [24, 26] and ISA-3D [38] was gathered from their reference publications (* data not published, ** data not available).

COMSTAT

ISA-3D

PHLIP


Operating systems

Linux, Windows

Windows 2000/XP*

Linux, OSX, Windows

Requirements:

Matlab license + toolboxes

Matlab license + toolboxes

Matlab license only

Reference

[24, 26]

[54]

http://www.phlip. org webcite

Availability

Obtained free upon request

Must attend workshop (1500$)

Free downloadable (open source)

Multi-channel capability

No

No

Yes

GUI

No

Yes*

Yes

CLSM support

- Leica

-COMSTAT

**

- Leica

- COMSTAT

- PHLIP-ML

(universal converter)

Preprocessing

None

- Image inversion

- Image inversion

- Carrier adjustment

- X- section resolution

Automatic thresholding

Yes

Yes

Yes (Otsu)

Noise reduction:

Connected volume filtration

**

Connected volume filtration

Image analysis parameters:

- Single channel (10)

-Single channel (20)

- Single channel (6)

- Two channel (2)

- All channel (5)

Result output

- Text format

- Text format

- HTML format

- XML format (PHLIP-ML)

- Text-format

Source code availability

Not available

Not available

Available (open source project)


Mueller et al. BMC Ecology 2006 6:1   doi:10.1186/1472-6785-6-1

Open Data