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Open Access Highly Accessed Research article

Resistance of rumen bacteria murein to bovine gastric lysozyme

María G Domínguez-Bello12*, M Andreína Pacheco2, Marie C Ruiz2, Fabián Michelangeli2, Matthias Leippe3 and Miguel A de Pedro4

Author Affiliations

1 Dept. Biology, University of Puerto Rico, Rio Piedras Campus, PO Box 23360, San Juan Puerto Rico 00931

2 Instituto Venezolano de Investigaciones Científicas, Centro de Biofísica y Bioquímica, A. postal 21827, Caracas 1020A, Venezuela

3 Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany

4 Laboratorio de Envolturas Bacterianas, Centro de Biología Molecular Severo Ochoa, CSIC, UAM, Madrid, Spain

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BMC Ecology 2004, 4:7  doi:10.1186/1472-6785-4-7

Published: 11 May 2004

Abstract

Background

Lysozymes, enzymes mostly associated with defence against bacterial infections, are mureinolytic. Ruminants have evolved a gastric c type lysozyme as a digestive enzyme, and profit from digestion of foregut bacteria, after most dietary components, including protein, have been fermented in the rumen. In this work we characterized the biological activities of bovine gastric secretions against membranes, purified murein and bacteria.

Results

Bovine gastric extract (BGE) was active against both G+ and G- bacteria, but the effect against Gram- bacteria was not due to the lysozyme, since purified BGL had only activity against Gram+ bacteria. We were unable to find small pore forming peptides in the BGE, and found that the inhibition of Gram negative bacteria by BGE was due to an artefact caused by acetate. We report for first time the activity of bovine gastric lysozyme (BG lysozyme) against pure bacterial cultures, and the specific resistance of some rumen Gram positive strains to BGL.

Conclusions

Some Gram+ rumen bacteria showed resistance to abomasum lysozyme. We discuss the implications of this finding in the light of possible practical applications of such a stable antimicrobial peptide.