Table 1

Key to grass identification at Sourhope using trnL PCR-RFLP. Fragment sizes were determined using nucleotide sequence data of the trnL PCR product of each reference species. Restriction enzyme(s) are followed by the fragment sizes expected from digestion with the enzyme(s). GenBank accession numbers are provided in brackets following the species name.

trnL PCR

Restriction digest 1

Restriction digest 2

Identity (accession number)

~500 bp

BfaI: 158/277/61

Agrostis capillaris (AY177347b)

BfaI: 439/61

Agrostis vinealis (AY177332)

>500 bp

NlaIV/DdeI: 57/53/187/317-329

Poa pratensis/trivialisa(AY177349b/AY177338)

NlaIV/DdeI: 57/53/200-204/298-300

XmnI: 197/45/367-370

Festuca rubra/ovinaa (AY177345b/AY177342)

XmnI: 197/45/178/154

Festuca juncifolia (AY177344)

NlaIV/DdeI: 57/53/505-536

Hsp92II: 125/435/86

Deschampsia flexuosa (AY177336)

Hsp92II: 125/405/86

Deschampsia caespitosa (AY177335)

Hsp92II: 336/200/86

Nardus stricta (AY177337)

Hsp92II: 563/86

Holcus lanatus (AY177348)

Hsp92II: 343/281

Luzula campestris (AY177333)

Hsp92II: 528/86

Phleum pratense (AY177339)

a can be separated by ITS PCR-RFLP with HinfI (Pp:335/293/76/17, Pt:293/207/127/76/17; Fr:337/297/76/17, Fo:293/230/107/76/17) b sequences from reference [6].

Ridgway et al. BMC Ecology 2003 3:8   doi:10.1186/1472-6785-3-8

Open Data