Open Access Research article

DNA barcoding of Northern Nearctic Muscidae (Diptera) reveals high correspondence between morphological and molecular species limits

Anaïs K Renaud12, Jade Savage2* and Sarah J Adamowicz3

Author Affiliations

1 Department of Entomology, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada

2 Department of Biological Sciences, Bishop’s University, Sherbrooke, Québec J1M 1Z7, Canada

3 Biodiversity Institute of Ontario & Department of Integrative Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada

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BMC Ecology 2012, 12:24  doi:10.1186/1472-6785-12-24

Published: 23 November 2012

Additional files

Additional file 1:

Specimen list. Name (pre and post-reassessment), Sample ID (voucher accession number), GenBank Accession Number, Process ID, Specimen repository and collection locality of 1303 specimens submitted for DNA amplification. Post-reassessment name provided only for specimens that yielded high quality sequences of at least 600 base pairs. Blue text indicates a successful amplification but low quality sequence (<600bp); red text indicates a failure to amplify; bolded text indicates a name change following the taxonomic reassessment.

Format: XLSX Size: 99KB Download file

Open Data

Additional file 2:

List of primers. Forward and reverse primers used to amplify COI sequences of muscid flies. The specific primers used for PCR and sequencing of each specimen are available through BOLD ( webcite).

Format: DOCX Size: 21KB Download file

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Additional file 3:

Neighbour-joining tree of pre-reassessment data set. Kimura 2-parameter NJ tree of 1133 high quality COI sequences (≥600bp) from 160 Muscidae species and undetermined material of Graphomya. The five putative lineages of Graphomya based on 1.2% threshold are highlighted in different colours. Bootstrap values based on 1000 replicates.

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Open Data