Open Access Research article

DNA barcoding of Northern Nearctic Muscidae (Diptera) reveals high correspondence between morphological and molecular species limits

Anaïs K Renaud12, Jade Savage2* and Sarah J Adamowicz3

Author Affiliations

1 Department of Entomology, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada

2 Department of Biological Sciences, Bishop’s University, Sherbrooke, Québec J1M 1Z7, Canada

3 Biodiversity Institute of Ontario & Department of Integrative Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada

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BMC Ecology 2012, 12:24  doi:10.1186/1472-6785-12-24

Published: 23 November 2012

Additional files

Additional file 1:

Specimen list. Name (pre and post-reassessment), Sample ID (voucher accession number), GenBank Accession Number, Process ID, Specimen repository and collection locality of 1303 specimens submitted for DNA amplification. Post-reassessment name provided only for specimens that yielded high quality sequences of at least 600 base pairs. Blue text indicates a successful amplification but low quality sequence (<600bp); red text indicates a failure to amplify; bolded text indicates a name change following the taxonomic reassessment.

Format: XLSX Size: 99KB Download file

Open Data

Additional file 2:

List of primers. Forward and reverse primers used to amplify COI sequences of muscid flies. The specific primers used for PCR and sequencing of each specimen are available through BOLD (http://www.boldsystems.org webcite).

Format: DOCX Size: 21KB Download file

Open Data

Additional file 3:

Neighbour-joining tree of pre-reassessment data set. Kimura 2-parameter NJ tree of 1133 high quality COI sequences (≥600bp) from 160 Muscidae species and undetermined material of Graphomya. The five putative lineages of Graphomya based on 1.2% threshold are highlighted in different colours. Bootstrap values based on 1000 replicates.

Format: PDF Size: 8.1MB Download file

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Open Data